For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.Read Tech Note
|MiSeq System||Up to 384 samples per run||Up to 2 x 300 bp|
|NextSeq 550 System||Up to 384 samples per run||Up to 2 x 150 bp|
|Nextera XT DNA Library Preparation Kit||Illumina DNA Prep|
|Method||16s rRNA Sequencing, Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing||Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing|
|Specialized Sample Types||Low-Input Samples, Single Cells||Blood, Not FFPE-Validated, Saliva|
The combination of Nextera XT and rapid sequencing with the MiSeq System provides a comprehensive DNA to data workflow in only 8 hours.
The Nextera XT Library Preparation Kit eliminates the need for library quantification before sample pooling and sequencing. Libraries of equivalent concentrations are created by employing bead-based sample normalization, as simple as pipetting 5 μl of each library to be sequenced.