TruSight Oncology 500 ctDNA
靶向血浆中多种体细胞变异类型（包括肿瘤突变负荷（TMB）和微卫星不稳定性（MSI））的检测。Read More...
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TruSight Oncology 500 ctDNA training
20045347
TruSight Oncology 500 ctDNA training
20045347
TruSight Oncology ctDNA是新一代测序（NGS）泛癌检测方法，支持在内部使用血浆进行全景变异分析（CGP）。
该panel的DNA内容与使用组织样本进行检测的对应产品（TruSight Oncology 500和TruSight Oncology 500 High-Throughput）相似，包含了关键的免疫肿瘤学（IO）生物标志物，这使其非常适合与珍贵组织样本结合使用或单独使用。
支持使用血浆的全景变异分析
通过集成的工作流程开展内部CGP
获得可靠的结果
解锁液体活检，包含有价值的IO信息
家族中的所有三种检测均旨在鉴定指南和临床试验中的重要生物标志物。
使用TruSight Oncology 500检测评估FFPE肿瘤组织的关键癌症生物标志物。
使用与TruSight Oncology 500检测相同的生物标志物和组织类型时，一次最多可批量处理192份样本。
使用TruSight Oncology 500 ctDNA检测分析血浆中的循环肿瘤DNA。
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NovaSeq 6000 System | 24 samples per run (S4 flow cell), 800M paired-end reads, 35,000x coverage | 2 × 150 bp |
| NovaSeq 6000 System | 8 samples per run (S2 flow cell), 800M paired-end reads, 35,000x coverage | 2 × 150 bp |
| TruSight Oncology 500 ctDNA | TruSight Oncology 500 | TruSight Oncology 500 High-Throughput | |
|---|---|---|---|
| Cancer Type | 泛癌种 | 泛癌种 | 泛癌种 |
| Content Specifications | 靶向选择523个基因（完整编码序列），panel总共1.94 Mb。 • 指南覆盖范围：广泛覆盖多种实体瘤的重要指南 • 免疫肿瘤学生物标志物覆盖范围：TMB和MSI* |
Targeted selection of DNA from 523 genes of interest, and RNA from 55
genes, for a total of 1.94Mb panel size. • Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types • Clinical Trials Coverage: Over 1,000 clinical trials • Immuno-oncology Biomarker Coverage: Biomarkers TMB and MSI included; also inclusive of HLA regions, POLE1 and POLD1*. |
Targeted selection of DNA from 523 genes of interest, and RNA from 55
genes, for a total of 1.94Mb panel size. • Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types • Clinical Trials Coverage: Over 1,000 clinical trials • Immuno-oncology Biomarker Coverage: Biomarkers TMB and MSI included; also inclusive of HLA regions, POLE1 and POLD1*. |
| Hands-On Time | Manual: ~10.5 hrs Automated: N/A |
Manual: ~10.5 hrs Automated: ~2.5 hrs |
Manual: ~10.5 hrs Automated: ~2.5 hrs |
| Input Quantity | 30 ng cfDNA（8-10 ml血浆） | 40 ng DNA, 40 ng RNA | 40 ng DNA, 40-80 ng RNA (need at least 2 mm3 FFPE tissue) |
| Method | 靶向DNA测序 , 靶向富集 | 靶向DNA测序 , 靶向RNA测序 , 靶向富集 | 靶向DNA测序 , 靶向RNA测序 , 靶向富集 |
| Nucleic Acid Type | DNA | DNA, RNA | DNA, RNA |
| Specialized Sample Types | Blood, 游离DNA | FFPE Tissue | FFPE Tissue |
| Species Category | 人类 | 人类 | 人类 |
| System Compatibility | NovaSeq 6000 | NextSeq 500 , NextSeq 550 , NextSeq 550Dx in Research Mode | NovaSeq 6000 |
| Technology | 测序 | 测序 | 测序 |
| Variant Class | 体细胞变异, 单核苷酸位点变异 (SNV), 基因融合, 拷贝数变异 (CNVs), 插入缺失 (indels) | Transcript Variants, 单核苷酸位点变异 (SNV), 基因融合, 拷贝数变异 (CNVs), 插入缺失 (indels), 转录变异 | Transcript Variants, 单核苷酸位点变异 (SNV), 基因融合, 拷贝数变异 (CNVs), 插入缺失 (indels), 转录变异 |
* Based on PierianDx Clinical Genomics Knowledgebase, as of March 2020.
Technical Note | PDF | 2 versions
Technical Note | PDF | 2 versions
Application Note | PDF | 5 versions
TruSight Oncology 500 ctDNA Gene List
product_file | EXCEL< 1 MB
Simplifying the process to identify applicable variants in oncology samples
Brochure | PDF3 MB
Data Sheet | PDF | 7 versions
