适用于MiniSeq系统的试剂和文库制备试剂盒包

一起订购MiniSeq试剂和文库制备试剂盒,能节省更多费用且更为方便。选择产品20005612并添加至购物车。

TruSight Cancer癌症相关基因探针集

这些专家定义的测序研究panel靶向与多种癌症易感性相关的94个基因和284个SNP。Read More...
选择产品

TruSight Cancer – Enrichment Oligos only (4 or 8 Enrichment Reactions)

FC-121-0202

TruSight Cancer MiniSeq Kit

20005612

Illumina Advantage Products

TG TruSight™ Cancer Sequencing Panel

TG-141-1002

配件产品
我需要什么配件?

Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)

FC-140-1101

TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)

FC-140-1102

TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)

FC-140-1103

TruSight Rapid Capture (24 indexes, 48 samples, 4 enrichments)

FC-140-1104

TruSight Rapid Capture (24 indexes, 96 samples, 8 enrichments)

FC-140-1105

TruSight Rapid Capture (96 indexes, 288 samples, 24 enrichments)

FC-140-1106

产品特色

这些预设计的即用型寡核苷酸是与癌症基因组学专家共同开发的,能让研究人员对与癌症易感性相关的各种基因和单核苷酸多态性(SNP)进行测序。

  • 靶向与癌症易感性相关的94个基因和284个SNP

TruSight Cancer包括与常见(如乳腺癌、直肠癌)和罕见癌症相关的基因。此外,该试剂盒还包括通过全基因组关联研究(GWAS)发现的与癌症相关的284个SNP。其内容根据业内专家对科学文献及其他高质量资源的筛选整理而选定。

TruSight Cancer测序panel提供靶向经过鉴定的目标区域的定制寡核苷酸。提供了足以进行四次富集反应的产品。TruSight Cancer与TruSight Rapid Capture兼容。

TruSight Cancer样本数据集

使用TruSight Cancer测序panel制备了6个人类参考样本。将这些文库在MiniSeq系统上以2x100 bp读长、带双端标签的配置,使用高通量试剂盒进行测序。总产量为5.2 Gb,其中95.8%的碱基分值大于等于Q30。

浏览BaseSpace Sequence Hub中的数据:

* 请注意,访问该数据需要登录BaseSpace Sequence Hub。

注册BaseSpace Sequence Hub账号

查看全部Illumina癌症研究panel

该产品是Illumina Advantage(TG)产品。Illumina Advantage大规模测序产品分批配送和测试,其保质期更长,且如有变动会预先通知,提高实验室的效率。

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
MiniSeq System Samples per run: mid output: 12, high output: 24 (based on > 20x coverage per target) Up to 2 × 150 bp
MiSeq System Samples per run (by reagent kit version): v2: 12, v3: 24 (based on > 20x coverage per target) Up to 2 × 150 bp
NextSeq 550 System Samples per run: mid output: 96, high output: 96 (based on > 20x coverage per target) Up to 2 × 150 bp

产品比较

TruSight Cancer癌症相关基因探针集 AmpliSeq for Illumina BRCA Panel
Assay Time 1.5 days 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer Type 泛癌种 Solid Tumor
Content Specifications Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2
Description Germline mutation detection research for common and rare cancers. Germline and somatic analysis studies of BRCA1 and BRCA2 .
Hands-On Time 5 hours < 1.5 hours
Input Quantity 50 ng DNA 1–100 ng (10 ng recommended per pool)
Method 靶向DNA测序 , 靶向富集 Amplicon Sequencing , 靶向DNA测序
Multiplexing Up to 96-plex 96 dual index combinations
Specialized Sample Types Not FFPE-Compatible Blood, FFPE Tissue
Variant Class 生殖系变异 体细胞变异, 单核苷酸多态性 (SNPs), 插入缺失 (indels), 生殖系变异

具体的方法工作流程范例

 

客户案例

From Arrays to NGS: How Chromosomal Genetics Evolved From Structure to Disease

Researchers from the MGZ Medical Genetic Center are using the Infinium CytoSNP-850K BeadChip and the TruSight Cancer Panel to analyze chromosomal abnormalities.

Read More

产品资料

TruSight Cancer Gene List

product_file | EXCEL< 1 MB

TruSight Cancer

Data Sheet | PDF< 1 MB

相关产品

Nextera Flex for Enrichment

广泛适用于各种应用的快速、集成的工作流程,能为定制panel、固定基因集的panel和全外显子组富集提供靶向重测序。


AmpliSeq for Illumina Custom DNA Panel

专为特异性靶点或感兴趣的基因组内容测序进行优化的靶向定制研究panel。


AmpliSeq for Illumina BRCA Panel

用于研究BRCA1BRCA2基因的体细胞和生殖系变异的靶向研究panel。


关于针对产品特定用途的生物标记物专利及其他专利的说明:
某些基因组变异(包括某些核酸序列)及其在特定应用中的用途可能受到专利保护。建议客户确定是否需要从拥有此类专利的第三方获得相应许可,以便在其特定应用中使用本产品。