AmpliSeq for Illumina TCR beta-SR (Short Read) Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples. It is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. You can use a single pool of multiplex PCR primers, library reagents, and sample barcodes to generate libraries from DNA/RNA extracted from FFPE, whole blood, fresh-frozen tissue, or fluorescence activated cell sorting (FACS)-sorted cells.
Key features include:
- High-Value Content
Obtain comprehensive coverage of TCR beta chain sequences
- Simple, Streamlined Workflow
Prepare sequencing-ready libraries in a single day from FFPE, whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS) cells
- Low Input Requirement
Generate high-quality sequencing libraries from as little as 10 ng of input RNA
The AmpliSeq for Illumina TCR beta-SR Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. It enables characterization of the hypervariable complementary determining region 3 (CDR3) of the TCR beta chain. This information can help immunology researchers understand immune function, autoimmune diseases, and immune-mediated adverse effects.
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