NOTE: An update to NextSeq Control Software (NCS 2.2.0) is required to run the NextSeq v2.5 sequencing reagent kits.

NextSeq 500/550 v2.5 Kits

These sequencing reagent kits offer increased stability and robustness over the v2 kit version.Read More...
Select Product(s)

NextSeq 500/550 High Output Kit v2.5 (75 Cycles)

20024906

Price
 
 

NextSeq 500/550 High Output Kit v2.5 (150 Cycles)

20024907

Price
 
 

NextSeq 500/550 High Output Kit v2.5 (300 Cycles)

20024908

Price
 
 

NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles)

20024904

Price
 
 

NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles)

20024905

Price
 
 
Illumina Advantage Products

TG NextSeq™ 500/550 High Output Kit v2.5 (75 Cycles)

20024911

Price
 
 

TG NextSeq™ 500/550 High Output Kit v2.5 (150 Cycles)

20024912

Price
 
 

TG NextSeq™ 500/550 High Output Kit v2.5 (300 Cycles)

20024913

Price
 
 

TG NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles)

20024909

Price
 
 

TG NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles)

20024910

Price
 
 

Product Highlights

NextSeq 500/550 v2.5 sequencing reagent kits deliver the power of high-throughput sequencing on a benchtop system with increased stability and robustness. These kits offer:

  • New v2.5 flow cell provides greater stability and robustness
  • Continued use of proven v2 reagent and buffer cartridges
  • Multiple options for both sequencing output and read length
  • Robust base calling and high signal-to-noise ratio
  • Consistent run to run performance

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Ready-to-use cartridges redefine ease-of-use
Preconfigured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in three ready-to-use cartridges (reagent, flow cell, and buffer). The kits offer simplified cartridge loading with the bleach and dual-indexing primers already in the cartridge.

Simply load user-prepared library pools directly into the NextSeq reagent cartridge. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

Frequently Purchased Together

Specifications

How to Achieve More Consistent Cluster Density on Illumina Sequencing Platforms

Achieving optimal cluster density is critical to high-quality sequencing on MiniSeq, MiSeq, NextSeq, and HiSeq 2500 Systems. Read more about how to achieve consistent cluster density in order to streamline data outputs.

Read Bulletin
How to Achieve More Consistent Cluster Density on Illumina Sequencing Platforms

Configurations for every application

Researchers have a wide range of solutions for different applications, from ChIP-Seq and RNA profiling to whole-genome sequencing, as shown in the table. NextSeq reagent kits provide optimized chemistry with paired-end sequencing capability built into every reagent kit.

 

NextSeq 500/550 Kits High Output
Up to 400 million clusters
Mid Output
Up to 130 million clusters

300 cycles
  • Whole Genome
  • de novo
  • Exome
  • Targeted Panels
  • Exome

150 cycles
  • Whole Transcriptome
  • mRNA-Seq
  • Exome
  • Whole Transcriptome
  • mRNA-Seq
  • Exome

75 cycles

 

  • Gene Expression Profiling
  • NIPT
 

†All NextSeq 500/550 kits are paired-end capable

The Illumina Community
The Illumina Online Community

Discuss best practices, troubleshoot, and learn about how others are using Illumina sequencers, library preparation kits, and data analysis to fuel their research. Collaborate with Illumina customers, moderators, and developers. 

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Product Literature

NextSeq 550 Sequencing System

Data Sheet | PDF2 MB

NextSeq 550 amplicon sequencing solution

Application Note | PDF< 1 MB

NextSeq 550 exome sequencing solution

Application Note | PDF< 1 MB

NextSeq 550 RNA-Seq solution

Application Note | PDF< 1 MB

NextSeq 550 System

Data Sheet | PDF< 1 MB

Manuals and Support Information

NextSeq 500/550 Kit Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All NextSeq 500/550 Kit Support 

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