TruSight Oncology 500 High-Throughput

Enabling comprehensive genomic profiling from FFPE samples, with added flexibility to batch up to 192 samples per flow cell. Read More...
Select Product(s)
What Products do I need?
Library Prep

TruSight Oncology 500 DNA High-Throughput Kit (48 Samples)

20040765

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (144 Samples)

20040767

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (24 Samples)

20040764

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples)

20040766

Price
 
 

Software Options

TruSight Oncology 500 DNA/RNA High-Throughput Kit (24 Samples), plus PierianDx

20040768

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (48 Samples), plus PierianDx

20040769

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples), plus PierianDx

20040770

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (144 Samples), plus PierianDx

20040771

Price
 
 

Index Adapters

IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20034701

Price
 
 

IDT® for Illumina® UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20034702

Price
 
 

Reagents

NovaSeq 6000 S4 Reagent Kit v1.5 (200 cycles)

20028313

Price
 
 

NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles)

20028315

Price
 
 

NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles)

20028318

Price
 
 

NovaSeq 6000 SP Reagent Kit v1.5 (200 cycles)

20040719

Price
 
 

NovaSeq 6000 SP Reagent Kit (200 cycles)

20040326

Price
 
 

NovaSeq 6000 S1 Reagent Kit (200 cycles)

20012864

Price
 
 

NovaSeq 6000 S2 Reagent Kit (200 cycles)

20012861

Price
 
 

NovaSeq 6000 S4 Reagent Kit (200 cycles)

20027466

Price
 
 

Services

Trusight Oncology 500 HT Training (DNA) - Customer Site

20042220

Price
 
 

Trusight Oncology 500 HT Training (DNA and RNA) - Customer Site

20042219

Price
 
 

Product Highlights

The TruSight Oncology 500 High-Throughput assay is built on the foundation of the TruSight Oncology 500 assay, with flexibility for higher sample throughput and batching options.

Consolidate Multiple Biomarker Assays into One

  • Pan-cancer biomarker content aligned with key guidelines and clinical trials
  • DNA + RNA assay targeting 523 genes for assessment of all DNA and RNA variant types, plus MSI and TMB
  • Increase chances of finding a positive biomarker by moving from individual biomarker assays to a single comprehensive NGS assay

Streamlined Sample-to-Results Workflow

  • Go from initial sample to results in 4-5 days

High Sample Throughput

  • Flexible sample batching allows you to run anywhere from 16 to 192 samples per sequencing flow cell on the NovaSeq 6000 System.
  • Automation kits and methods available for reduced hands-on time and scalability
  • Easily translate raw data into a final interpretation report

Achieve Highly Confident Results

  • Hybrid-capture chemistry combined with sophisticated bioinformatics, leading to high analytical specificity and sensitivity
  • Inclusive of unique molecular indexes (UMIs) for high sensitivity in variant detection
  • Based on proven Illumina SBS sequencing technology

Enable In-House Comprehensive Genomic Profiling

  • Keep samples and data in house, for more control and ability to build a database
  • A future-proof assay with emerging biomarkers already included
Learn more about TruSight Oncology 500 Family

TruSight Oncology 500 Product Family

All three assays in the family are designed to identify key biomarkers in guidelines and clinical trials.

TruSight Oncology 500 Assay

Assess key cancer biomarkers from FFPE tumor tissue with the TruSight Oncology 500 Assay.

TruSight Oncology 500 High-Throughput Assay

Batch up to 192 samples at a time while using the same biomarkers and tissue type as the TruSight Oncology 500 assay.

TruSight Oncology 500 ctDNA Assay

Analyze circulating tumor DNA from plasma with the TruSight Oncology 500 ctDNA assay.

Frequently Purchased Together

Specifications

Product Comparison

TruSight Oncology 500 High-Throughput TruSight Oncology 500 TruSight Oncology 500 ctDNA
Cancer Type Pan-Cancer Pan-Cancer Pan-Cancer
Content Specifications Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size.
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical Trials Coverage: Over 1,000 clinical trials
• Immuno-oncology Biomarker Coverage: Biomarkers TMB and MSI included; also inclusive of HLA regions, POLE1 and POLD1*.
Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size.
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical Trials Coverage: Over 1,000 clinical trials
• Immuno-oncology Biomarker Coverage: Biomarkers TMB and MSI included; also inclusive of HLA regions, POLE1 and POLD1*.
Targeted selection of 523 genes (full coding sequence) for a total of 1.94Mb panel size.
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Immuno-oncology Biomarker Coverage: TMB and MSI*
Hands-On Time ~10.5 hours ~10.5 hours ~10.5 hours
Input Quantity 40 ng DNA, 40-80 ng RNA (need at least 2 mm3 FFPE tissue) 40 ng DNA, 40 ng RNA 30 ng cfDNA (8-10 ml of plasma)
Method Target Enrichment , Targeted DNA Sequencing , Targeted RNA Sequencing Target Enrichment , Targeted DNA Sequencing , Targeted RNA Sequencing Target Enrichment , Targeted DNA Sequencing
Nucleic Acid Type DNA, RNA DNA, RNA DNA
Sample Throughput 16–192 samples per flow cell
Specialized Sample Types FFPE Tissue FFPE Tissue Blood, Cell-Free DNA
Species Category Human Human Human
System Compatibility NovaSeq 6000 NextSeq 500 , NextSeq 550 , NextSeq 550Dx in Research Mode NovaSeq 6000
Technology Sequencing Sequencing Sequencing
Variant Class Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Somatic Variants

Based on PierianDx Clinical knowledgebase, as of March 2020

Method-Specific Workflow Example

 

Supporting Data and Figures

 

Related Products

TruSight Oncology 500 ctDNA

Assay targeting multiple somatic variant types from plasma, including microsatellite instability (MSI) and tumor mutational burden (TMB).


TruSight Oncology 500

Assay targeting multiple variant types, including microsatellite instability (MSI) and tumor mutational burden (TMB).