TruSight Cardio Sequencing Kit for NextSeq v2 (FC-141-1011) has been discontinued; the recommended replacement is TruSight Cardio Sequencing Kit for NextSeq v2.5 (20035190). The library prep components of the kit have not changed. Please contact your local sales representative for additional information on this change.
|Instrument||Recommended Number of Samples||Read Length|
|MiSeq System||12 samples per run with v2 reagents (based on 300x mean coverage of targeted content)||2 × 150 bp (max recommended)|
|NextSeq 550 System||48 samples per run (mid output; based on 300x mean coverage of targeted content)||2 × 150 bp (max recommended)|
|TruSight Cardio Sequencing Kit||TruSight One Sequencing Panels||TruSight Inherited Disease|
|Content Specifications||575 Kb genomic content (174 genes)||TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).
|2.25 Mb genomic content (552 genes)|
|Description||Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions.||Fixed research panels targeting exonic regions that harbor disease-causing variants.||Fixed research panel focused on severe pediatric onset disorders.|
|Input Quantity||50 ng DNA||50 ng DNA||50 ng DNA|
|Method||Target Enrichment , Targeted DNA Sequencing||Target Enrichment , Targeted DNA Sequencing||Target Enrichment , Targeted DNA Sequencing|
|Multiplexing||Up to 96-plex||Up to 96-plex||Up to 96-plex|
|Specialized Sample Types||Not FFPE-Compatible||Not FFPE-Compatible||Not FFPE-Compatible|
The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.
* Data on file. Illumina, Inc. 2015.