环境宏基因组学是基于环境样品中的DNA分析,对微生物群落中生物体的研究。
具体包括分析来自深海火山口的水样中的微生物种群,或来自人为环境(如活性矿物矿井)的土壤样品。环境宏基因组学的研究数据被用于农业微生物组分析、生态修复,或其他的生物学研究。
在新一代测序(NGS)出现之前,环境宏基因组学作为一个领域是极其有限的。NGS让研究人员能够分析复杂样品中的整个微生物群落,发现新的生物,并探索不断变化的条件下微生物种群的动态性质。
根据希望获得的信息不同,环境宏基因组学研究所用的NGS方法也不同,具体包括:
The Earth BioGenome Project is a confederated network of partner organizations and affiliated projects that have a common goal of sequencing and annotating the genomes of all 1.5 million known species of eukaryotes on the planet in 10 years.
Read ArticleThis metagenomic shotgun sequencing workflow enables accurate DNA detection from soil.
Read ArticleNGS and environmental DNA (eDNA) metabarcoding enable researchers to monitor a variety of ecosystems accurately.
Read InterviewAn epic ocean expedition sequenced salt water samples from around the globe to survey plankton diversity in marine ecosystems.
Read InterviewLearn about the use of NGS in efforts to map Stockholm’s subway microbiome and study the metagenome of urban environments in more than 67 cities worldwide.
Read Article环境DNA(eDNA)测序是一种迅速兴起的方法,可研究生物多样性并监测生态系统的改变。环境DNA的鸟枪法测序有助于研究可能在样本中大量存在的物种,例如细菌或小型真核生物。
了解更多University of Wisconsin-Madison researchers explore a microbe-cycling hypothesis with tree sloths and paired-end 16S amplicon sequencing.
Researchers use NGS to identify and understand the constellation of microbes that deliver flavor to cheese and wine.