过去,基于芯片的单核苷酸多态性(SNP)筛查方法是分析许多植物和动物的性状并将其与基因组区域建立关联的首选方法。由于测序成本持续下降,研究人员正在开发利用新一代测序(NGS)技术进行基因分型的新方法。
基于测序的基因分型或新一代基因分型是一种用于发现新的动植物SNP位点并进行基因分型研究的基因筛查方法。对于某些应用(例如基因型筛查及基因定位),基于测序的基因分型为基因变异研究提供了一种比芯片成本更低的替代方法。
基于测序的基因分型是一种经济高效的方法,适用于具有复杂基因组或资源有限的群体。使用的技术包括基于扩增子的靶向测序、以杂交为基础的富集测序以及基于序列的限制性酶简化基因分型。
Targeted custom research panels optimized for specific targets or genomic content of interest.
An on-premises software solution for creating sequencing runs, monitoring run status, and analyzing data.
AgResearch uses genotyping by sequencing to overcome cost and resource hurdles associated with genomic selection of minor livestock species, such as the Meredith Dairy goat herd.
Read InterviewIllumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating approximately 90% of global sequencing data.*
Illumina offers integrated sequencing workflows that simplify the entire process, from library preparation to data analysis and biological interpretation.
Click on the below to view products for each workflow step.
An easy-to-use online software tool that provides dynamic feedback to optimize probe designs.
AmpliSeq for Illumina Custom PanelsCreate custom targeted sequencing panels optimized for content of interest.
Library preparation for small genomes (bacteria, archaea, viruses), amplicons, and plasmids in less than 90 minutes.
Determine the best kit for your needs based on project type, starting material, and method or application.
Speed and simplicity for targeted and small genome sequencing.
NextSeq SeriesFlexible power for whole-genome, transcriptome, and targeted resequencing.
Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.
Platform Comparison ToolCompare sequencing platforms and identify the best system for your lab and applications.
Sequencing ReagentsFind kits that include sequencing reagents, flow cells, and/or buffers tailored to each Illumina sequencing system.
An on-premises software solution for creating sequencing runs, monitoring run status, and analyzing data.
BaseSpace BWA Aligner AppAligns samples (consisting of FASTQ files) to a reference genome.
BaseSpace Whole-Genome Sequencing AppQuickly extracts biological information from whole-genome sequences, using Isaac alignment and variant calling.
The Illumina genomics computing environment for NGS data analysis and management.
BaseSpace Correlation EngineA growing library of curated genomic data to support researchers in identifying disease mechanisms, drug targets, and biomarkers.
对于小型基因组(例如果蝇)或广受关注的研究物种(例如拟南芥),可以使用参考相关的标准全基因组测序或重测序方法完成基因分型和变异筛查。浅度测序可用于进化分析或对比分析。
了解详情NGS technology can benefit agricultural studies of plants and animals, whether it is used for de novo sequencing, transcriptome analysis, genotyping by sequencing, or metagenomics. Learn more about plant and animal sequencing.
The application of genomics in commercial agriculture is helping breeders and researchers perform trait screening, parentage testing, and more. Learn more about commercial agriculture and genomics.
This application spotlight describes the advantages and considerations of several sequence-based genotyping research methods.
Scientists developed Genotyping-in-Thousands by Sequencing (GT-Seq) to genotype thousands of fish simultaneously.
Amplicon sequencing is a highly targeted approach that researchers can use to genotype large genomes.
*Data calculations on file. Illumina, Inc., 2015