Introduction to Methylation Array Analysis
DNA methylation plays an important and dynamic role in regulating gene expression. It allows cells to acquire and maintain a specialized state, suppresses the expression of viral and nonhost DNA elements, and facilitates response to environmental stimuli. Aberrant DNA methylation (hyper- or hypomethylation) and its impact on gene expression have been implicated in many normal and aberrant biological processes.
Infinium Methylation Arrays
Our Infinium MethylationEPIC and Mouse Methylation BeadChips allow you to interrogate 850,000 or 285,000 markers, respectively, across the genome at single-nucleotide resolution.
Human MethylationEPIC BeadChip
Powered by the Infinium assay, the MethylationEPIC BeadChip is ideal for epigenome-wide association studies (EWAS), tumor profiling, and various cell biology experiments. It offers comprehensive, expeert-selected coverage, including 99% of RefSeq genes, 95% of CpG islands, high coverage of enhancer regions, and other content categories. With > 90% of the original content from the Infinium HumanMethylation450K covered, the MethylationEPIC kit supports the next generation of epigenetics research.
Mouse Methylation BeadChip
Also leveraging the Infinium assay, the Mouse Methylation BeadChip can be used in EWAS and other experiments probing the methylome in healthy and diseased tissues. Much like the MethylationEPIC BeadChip, the Mouse Methylation BeadChip offers genome-wide coverage, with probes targeted to CpG islands, enhancer regions, transcription start sites, and other important regions of the methylome.
甲基化芯片的优势
MethylationEPIC BeadChip结合了全面的覆盖范围和高通量能力，是EWAS的理想选择。其他优势包括：
- 全面覆盖整个基因组（超过850,000个甲基化位点）
- CpG岛
- 非CpG和差异甲基化位点
- FANTOM5增强子
- ENCODE开放染色质
- ENCODE转录因子结合位点
- miRNA启动子区域
- 检测重现性
- 技术平行重现性为98%
- 相同的样本在经典的HumanMethylation450K芯片和MethylationEPIC芯片上的重现性为98%
- 90%以上的HumanMethylation450K内容
- 用户友好的一体化工作流程
- 与福尔马林固定、石蜡包埋样本兼容
甲基化芯片工作流程
MethylationEPIC BeadChip具有用户友好的一体化工作流程，能以低样本起始量（低至250 ng）同时处理多达96个样本。
该甲基化芯片能够在单个CpG位点的水平上提供定量测量，可用于正常及FFPE样本，为深入了解表观遗传变化提供了强大的检测分辨率。
点击下方的查看工作流程各步骤所使用的产品。
MethylationEPIC BeadChip
无需PCR，样本起始量低（低至250 ng），该试剂盒使用Infinium I和Infinium II检测化学技术。
Infinium Mouse Methylation BeadChip
Features > 285k markers across the methylome for high resolution epigenetic analyses of diverse murine strains.
iScan系统
支持高通量的BeadChip处理，可快速准确地扫描成百上千个样本。
NextSeq 550 System
Perform next-generation sequencing and methylation array scanning all on one platform.
GenomeStudio软件和甲基化模块
毫不费力的甲基化差异分析，并显示染色体坐标、GC百分比、CpG岛的位置和甲基化ß值。
相关解决方案
复杂疾病研究
甲基化芯片研究使研究人员可以了解复杂疾病中的功能机制。Illumina为研究这些疾病的研究人员提供了全面的解决方案。深入了解复杂疾病研究。
表观遗传学和癌症
Illumina芯片和测序解决方案是检测甲基化模式和表观遗传学变化的理想选择，有助于了解致癌通路和癌症进展。深入了解癌症表观遗传学。
