货号为RS-122-2501、RS-122-2502和RS-122-2503的产品配置有更改。在新的配置中,文库制备试剂和标签接头均可单独购买。可单独购买的组件列表可在“选择产品”部分中找到。
TruSeq Stranded Total RNA with Ribo-Zero Globin采用迅捷高效的测序文库制备工作流程,能从血液来源的总RNA中得到清晰、完整的转录组视图。
这些试剂盒集Ribo-Zero核糖体RNA去除方法与RNA测序(RNA-Seq)技术的优点于一体,可对人类、小鼠或大鼠样本进行全转录组分析。该技术能:
对血液来源的RNA进行全转录组分析需要去除两种在全血中含量较高的RNA——核糖体RNA(包括细胞质rRNA和线粒体rRNA)和球蛋白mRNA。
传统去除方法需要两个步骤才能完成。这意味着需要的试剂更多、工作流程更长、损失的RNA更多。TruSeq Stranded Total RNA with Ribo-Zero Globin利用了Ribo-Zero测序化学技术,能通过一个简单、快速的步骤高效去除两种含量较高的RNA。
仪器 | 推荐的样本数 | 读长 |
---|---|---|
NextSeq 550 System | RNA Profiling: 6-20 samples per run (based on 20 million reads per
sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample) |
2 x 75 bp 2 x 75 bp |
HiSeq 2500 System | RNA Profiling: 30-200 samples per run (dual flow cell; based on 20
million reads per sample) Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample) |
2 x 75 bp 2 x 75 bp |
NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4:
768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. |
≤ 2 × 100 bp ≤ 2 × 100 bp |
TruSeq Stranded Total RNA with Ribo-Zero Globin | Illumina Stranded Total RNA Prep with Ribo-Zero Plus | |
---|---|---|
Assay Time | 11.5 hours | 约7小时 |
Automation Capability | 液体处理机器人 | 液体处理机器人 |
Content Specifications | 捕获编码RNA以及多种形式的非编码RNA。 | 捕获编码RNA以及多种形式的非编码RNA。 |
Description | 用于血液来源RNA的全转录组测序研究。去除细胞质rRNA、线粒体rRNA和珠蛋白mRNA。 | 用于多种类型样本的全转录组测序研究。包含Ribo-Zero plus模块,一个试管即可去除多个物种的转录本。 |
Hands-On Time | 5.5 hours | <3小时 |
Input Quantity | 0.1–1 ug血液来源的高质量纯化总RNA | 对于标准质量RNA样本,可使用1–1000 ng的总RNA。对于FFPE样本,建议最少使用10 ng的总RNA,以获得理想效果。 |
Mechanism of Action | 基于微珠的rRNA去除、cDNA合成和PCR | 酶法rRNA去除,连接接头和标签 |
Method | 全转录组测序 | 全转录组测序 |
Multiplexing | 高通量试剂盒:使用组合双标签或唯一双标签序列制备96个带标签的样本。, 低通量试剂盒:混合最多12个样本。或混合24个样本(组A和B一起) | 多达384个唯一双标签序列(UDI) |
Specialized Sample Types | Blood | FFPE Tissue, Low-Input Samples |
Species Category | 人类, 大鼠, 小鼠 | Virus, 人类, 大鼠, 小鼠, 细菌 |
Species Details | rRNA Removal: Depletes human, mouse, and rat rRNA (cytoplasmic and mitochondrial) and globin mRNA | 包含Ribo-Zero Plus,用于去除多个物种的转录本,包括人类细胞质和线粒体rRNA、小鼠rRNA、大鼠rRNA、细菌革兰氏+/- rRNA和人β珠蛋白转录本。 |
Technology | 测序 | 测序 |
The product configuration for Cat. No. RS-122-2501, RS-122-2502, and RS-122-2503 has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Data Sheet | PDF | 5 versions
TruSeq Stranded Total RNA Sample Preparation User-Supplied Consumables and Equipment Documentation
Sequencing Library qPCR Quantification Guide Documentation
TruSeq HT Dual Index Quick Reference Card Documentation
TruSeq Sample Prep Best Practices Guide Documentation
TruSeq Stranded Total RNA Sample Preparation LS EUC and LTF Documentation
Illumina Experiment Manager User Guide Documentation
TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
TruSeq Stranded Total RNA Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA Sample Prep HS EUC and LTF Documentation
TruSeq Stranded Total RNA Sample Prep Guide Documentation
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Stranded Total RNA Reference Guide Documentation
TruSeq Stranded Total RNA Dual Index Product Insert Documentation
自定义协议选择器
Generates customized, end-to-end instructions
有限标签使用许可:TruSeq Stranded Total RNA with Ribo-Zero Globin工作流程的产品及其使用受Max Planck Gesellschaft所拥有且独家授权给New England Biolabs, Inc.并再授权给Illumina, Inc.的一个或多个已公告和/或待批准的美国和外国专利申请的约束。向Illumina, Inc.、其关联机构或其授权经销商和分销商购买此产品,即向买方转让使用买方所购数量产品和产品组件的权利(不论买方是学术性还是盈利性实体),该权利不可转让。购买本产品不会获得上述专利或专利申请权利请求项下的产品生产许可。买方不得向第三方出售或以其他方式转让此产品及其组件,亦不得以其他方式将此产品用于下商业用途:(1)在生产中使用此产品或其组件;或(2)出于治疗或预防疾病的目的,在人或动物身上使用此产品或其组件。