The product configuration for Cat. No. RS-122-2501, RS-122-2502, and RS-122-2503 has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
TruSeq Stranded Total RNA with Ribo-Zero Globin delivers a clear and comprehensive view of the transcriptome from blood-derived total RNA, with fast, efficient sequencing library preparation.
These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:
Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.
Traditional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.
Find automation vendors with robotic systems compatible with this product
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | RNA分析：每次运行6–20个样本（基于每个样本2000万条read） 转录组分析：每次运行2–8个样本（基于每个样本5000万条read） |
2 x 75 bp 2 x 75 bp |
| HiSeq 2500 System | RNA分析：每次运行30–200个样本（双流动槽；基于每个样本2000万条read） 转录组分析：每次运行12–80个样本（双流动槽；基于每个样本5000万条read） |
2 x 75 bp 2 x 75 bp |
| NovaSeq 6000 System | RNA分析（每次运行的样本，双流动槽）：S1：160，S2：320，S4：768。受标签组合的限制（双重）。基于2000万条read。 转录组分析（每次运行的样本，双流动槽）：S1：64，S2：128，S4：400。受标签组合的限制（双重）。基于5000万条read。 |
≤ 2 × 100 bp ≤ 2 × 100 bp |
| TruSeq Stranded Total RNA with Ribo-Zero Globin | TruSeq Stranded Total RNA | AmpliSeq for Illumina Transcriptome Human Gene Expression Panel |
|---|
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
TruSeq Stranded Total RNA gives excellent coverage across the top 1,000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis = percent coverage of combined reads.
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Data Sheet | PDF | 5 versions
TruSeq Stranded Total RNA Sample Preparation User-Supplied Consumables and Equipment Documentation
Sequencing Library qPCR Quantification Guide Documentation
TruSeq HT Dual Index Quick Reference Card Documentation
TruSeq Sample Prep Best Practices Guide Documentation
TruSeq Stranded Total RNA Sample Preparation LS EUC and LTF Documentation
Illumina Experiment Manager User Guide Documentation
TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
TruSeq Stranded Total RNA Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA Sample Prep HS EUC and LTF Documentation
TruSeq Stranded Total RNA Sample Prep Guide Documentation
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Stranded Total RNA Reference Guide Documentation
TruSeq Stranded Total RNA Dual Index Product Insert Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
Limited Use Label License: The TruSeq Stranded Total RNA with Ribo-Zero Globin Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
