TruSeq Stranded mRNA
从mRNA制备测序文库，获得清晰的编码转录组视图以及链特异性信息。
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以前名为TruSeq Stranded mRNA Library Prep Kit的产品（货号RS-122-2101、RS-122-2102和RS-122-2103）现更名为TruSeq Stranded mRNA。该产品的配置已更改。在新的配置中，文库制备试剂和标签接头均可单独购买。可单独购买的组件列表可在“选择产品”部分中找到。
TruSeq Stranded mRNA为编码转录组分析提供了经济的一体化可扩展解决方案，可兼容多种样本。
可精确测量mRNA链方向，用于检测反义转录、增强转录本注释和提高比对效率。高覆盖度均一性让诸如替代转录物、基因融合和等位基因特异性表达的发现变得更为容易。
通过链的信息来鉴别给定RNA转录本是由两条DNA链中哪一条衍生而来的。该信息提高了转录本注释的可信度，特别是对于非人类样本。鉴别链的起源增加了比对read的百分比，降低了每个样本的测序成本。
工作流程能够可靠分析标准和低质量样本，且兼容多种研究设计。
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 13–40 samples per run (based on 10 million reads per
sample) Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample) |
2 x 75 bp 2 x 75 bp |
| HiSeq 2500 System | RNA Profiling: 60–400 samples per run (dual flow cell; based on 10
million reads per sample) Transcriptome Analysis: 24–160 samples per run (dual flow cell; based on 25 million reads per sample) |
2 x 75 bp 2 x 75 bp |
| NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 320, S2:384,
S4:768. Limited by index combinations (dual). Based on 10 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 128, S2: 256, S4: 768. Limited by index combinations (dual). Based on 25 million reads. |
≤ 2 × 100 bp ≤ 2 × 100 bp |
| TruSeq Stranded mRNA | Illumina Stranded mRNA Prep | Illumina RNA Prep with Enrichment | |
|---|---|---|---|
| Automation Capability | 液体处理机器人 | 液体处理机器人 | 液体处理机器人 |
| Method | mRNA Sequencing | mRNA Sequencing | mRNA Sequencing , 靶向RNA测序 , 靶向富集 |
| Nucleic Acid Type | RNA | RNA | RNA |
| Species Category | Bovine, Mammalian, Mouse, Rat, 人类 | Bovine, Mammalian, Mouse, Rat, 人类 | Virus, 人类 |
| Strand Specificity | Stranded | Stranded | Non-Stranded |
| System Compatibility | Genome Analyzer IIx, HiSeq 1000, HiSeq 1500, HiSeq 2000, HiSeq 2500 , HiSeq 3000 , HiSeq 4000 , MiSeq , NextSeq 2000 , NextSeq 500 , NextSeq 550 , NovaSeq 6000 | HiSeq 2500 , HiSeq 3000 , HiSeq 4000 , NextSeq 1000 , NextSeq 2000 , NextSeq 500 , NextSeq 550 , NovaSeq 6000 | HiSeq 2500 , HiSeq 3000 , HiSeq 4000 , iSeq 100 , MiniSeq , MiSeq , NextSeq 1000 , NextSeq 2000 , NextSeq 500 , NextSeq 550 , NovaSeq 6000 |
| Technology | 测序 | 测序 | 测序 |
| Variant Class | Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 | Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 | Novel Transcripts, Transcript Variants, 单核苷酸多态性 (SNPs), 基因融合, 转录变异 |
The product previously known as the TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, RS-122-2102, and RS-122-2103) is now called TruSeq Stranded mRNA. The product configuration has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
图2来自TruSeq Stranded mRNA and Total RNA Library Prep Kit数据表，2015年12月
FFPE组织的技术平行分析显示出高度的一致性，表明文库制备的性能稳定。轴为log2(FPKM)。R2值如图所示。
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Data Sheet | PDF | 5 versions
TruSeq Stranded mRNA Checklist Documentation
TruSeq Stranded mRNA Documentation
TruSeq Stranded mRNA Reference Guide Documentation
TruSeq Stranded mRNA Consumables & Equipment Documentation
自定义协议选择器
Generates customized, end-to-end instructions
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.
此产品及其使用受Max Planck Gesellschaft所拥有且独家授权给New England Biolabs, Inc.并再授权给Illumina, Inc.的一个或多个已公告和/或待批准的美国和外国专利申请的约束。向Illumina, Inc.、其关联机构或其授权经销商和分销商购买此产品，即向买方转让使用买方所购数量产品和产品组件的权利（不论买方是学术性还是盈利性实体），该权利不可转让。购买此产品并不会转让前述专利或专利申请中针对产品生产的任何权利要求项下的许可。买方不得向第三方出售或以其他方式转让此产品及其组件，亦不得以其他方式将此产品用于下商业用途：（1）在生产中使用此产品或其组件；或（2）出于治疗或预防疾病的目的，在人或动物身上使用此产品或其组件。
