TruSeq DNA Exome

此试剂盒提供经济实惠的外显子组测序解决方案,在各种read深度下都能提供卓越的靶点覆盖度。Read More...
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TruSeq Exome Kit(24个样本)

20020614

TruSeq® Exome Kit(96样本)

20020615

Library Prep

TruSeq® DNA Library Prep for Enrichment(24样本)

20020181

TruSeq® DNA Library Prep for Enrichment(96样本)

20020182


Index Adapters

TruSeq DNA CD Indexes(96标签,96样本)

20015949

TruSeq DNA Single Indexes Set A(12标签,24样本)

20015960

TruSeq DNA Single Indexes Set B(12标签,24样本)

20015961

IDT for Illumina – TruSeq DNA UD Indexes(24标签,96样本)

20020590

IDT for Illumina – TruSeq DNA UD Indexes(96标签,96样本)

20022370

配件产品

Illumina游离接头阻断试剂(12次反应)

20024144

Illumina游离接头阻断试剂(48次反应)

20024145

Product Highlights

TruSeq Exome Kit(原名为TruSeq Exome Library Prep Kit)是一种经济实惠的文库制备和外显子组富集解决方案,可提供:

  • 用于低成本外显子组测序的模块化产品
  • 机械剪切和TruSeq富集技术可实现均匀覆盖,得到高匹配率的测序read
  • 完全受支持的工作流程解决方案,可简化外显子组测序
经济高效的外显子组测序

TruSeq Exome Kit支持12重预富集文库混合,每个流动槽通道可对多达12个文库进行测序,可最大限度地提高测序通量并进行变异鉴定。每次运行中测序的外显子组更多,让研究人员能最大限度地利用经费。

了解有关计算覆盖度估计值的更多信息,请参阅测序覆盖度计算器

成熟的TruSeq方法保证高数据质量

TruSeq Exome Kit可获得匹配率≥80%的测序read,实现高效、经济的外显子组测序。通过将TruSeq Exome Kit与使用边合成边测序(SBS)技术测序的Illumina系统结合起来,研究人员能鉴别出真正的编码变异,并最大限度地减少假阳性和假阴性结果。

方便、完整的流程方案

Illumina提供了集成且受支持的工作流程解决方案,指导研究人员完成从文库制备到分析的整个过程。外显子组测序工作流程的所有部分作为整体进行设计、优化和分析验证。专业的Illumina科学家将为流程的每一环节提供技术和现场支持。

Illumina现提供模块化产品的预订,让您的工作流程变得灵活。

查看兼容该产品的自动化系统的供应商

Illumina–IDT外显子组富集解决方案

Illumina现已与IDT合作,共同销售采用xGen® Exome Research Panel v1.0(由429,826个单独合成且质量受控的xGen Lockdown® Probe组成)的联合外显子组富集解决方案。本panel涵盖人类基因组的39 Mb靶标区域(19,396个基因),并覆盖51 Mb的端到端平铺空间。xGen阻断寡核苷酸(货号1075474、1075475或1075476,用于TruSeq文库),和xGen Lockdown Reagents(货号1072280或1072281)。

了解有关IDT xGen Exome Research Panel的更多信息
Illumina和IDT在NGS多重分析和外显子组富集领域合作

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System Samples per run: mid output: 3, high output: 12 (based on 50 million reads, 50× coverage, 4 Gb of data) 2 × 75 bp (max recommended)
HiSeq 2500 System Samples per run (dual flow cell): rapid run: 24, high output: 156 (based on 50 million reads, 50× coverage, 4 Gb of data) 2 × 75 bp (max recommended)
NovaSeq 6000 System Samples per run (dual flow cell): S1: 160, S2: 320, S4: 769 (based on 50 million reads, 50× coverage, 4 Gb of data) ≤ 2 × 100 bp

产品比较

TruSeq DNA Exome Nextera DNA Exome AmpliSeq for Illumina Exome Panel Nextera Flex for Enrichment
Assay Time 2.5 days < 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) ~6.5 hours
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). > 97% of CCDS Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
Description A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time. A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.
Hands-On Time 6 hours < 1.5 hours ~2 hours
Input Quantity 100 ng genomic DNA 50 ng minimum 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).
Mechanism of Action Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes Multiplex PCR Bead-bound transposomes and hybrid-capture chemistry
Method Exome Sequencing Exome Sequencing Amplicon Sequencing , Exome Sequencing Custom Sequencing, Exome Sequencing , 靶向DNA测序 , 靶向富集
Multiplexing Up to 12-plex enrichment 96 dual index combinations Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.
On-Target Reads >80% >80% ≥85% (for exome panel)
Specialized Sample Types FFPE Tissue Low-Input Samples 不兼容FFPE Blood, FFPE Tissue, Saliva
Species Category 人类 人类 人类 Other, 人类
Uniformity >85% (% coverage at 20x for 4 Gb) >90% (% coverage at 20x for 4 Gb) ≥90% (% coverage at 20x for 4 Gb; for exome panel)

具体的方法工作流程范例

 

客户案例

支持数据和图像

靶向外显子组富集
在靶read的百分比

TruSeq Exome Kit可获得匹配率≥80%的测序read,实现高效、经济的外显子组测序。

 
TruSeq Exome Kit (Pre-Assembled Kits)
Catalog Number Total Samples Number of Enrichment Reactions Index Adapters
20020614 24 8 24 single indexes (Set A and Set B)
20020615 96 8 96 combinatorial dual indexes

相关产品

Nextera DNA Exome

This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.


Nextera Flex for Enrichment

广泛适用于各种应用的快速、集成的工作流程,能为定制panel、固定基因集的panel和全外显子组富集提供靶向重测序。


TruSight One Sequencing Panels

此Panel专注于含致病变异的外显子区域。其可让实验室通过一个实验分析方法代替多种不同的分子实验分析方法。