*Offer valid for library prep kit only. Index adapters and panels are not included in this promotion.
Illumina Stranded Total RNA Library Prep Training
|NextSeq 550 System||Mid output: 2
High output: 8
(based on 50M reads per sample)
|2 × 75 bp|
|NextSeq 2000 System||Whole Transcriptome Analysis:
8 (P2)-20 (P3) samples per run (based on 50M reads per sample)
|2 × 75 bp|
|NovaSeq 6000 System||Whole Transcriptome Analysis:
samples per run (based on 50M reads per sample)
|2 × 75 bp|
|Illumina Stranded Total RNA Prep with Ribo-Zero Plus||TruSeq Stranded Total RNA||TruSeq Stranded Total RNA with Ribo-Zero Globin|
|Assay Time||~7 hours|
|Content Specifications||Captures coding RNA plus multiple forms of non-coding RNA||Captures coding RNA plus multiple forms of non-coding RNA|
|Description||For whole-transcriptome sequencing studies of multiple sample types. Includes Ribo-Zero plus module for single-tube depletion of abundant transcripts from multiple species||For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA.|
|Input Quantity||1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples||0.1 – 1 ug high-quality purified total RNA from blood|
|Mechanism of Action||Enzymatic rRNA depletion, ligation-based addition of adapters and indexes||Bead-based rRNA depletion, cDNA synthesis, and PCR|
|Method||Whole-Transcriptome Sequencing||Whole-Transcriptome Sequencing||Whole-Transcriptome Sequencing|
|Multiplexing||多达384个唯一双标签序列（UDI）||High-throughput kits: Prepare 96 indexed samples using either combinatorial dual indexes or unique dual indexes, Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together|
|Species Category||Bacteria, Mouse, Rat, Virus, 人类||Mouse, Rat, 人类||Mouse, Rat, 人类|
Top- Multispecies ribodepletion with Illumina Stranded Total RNA Prep with Ribo-Zero Plus. Illumina Stranded Total RNA Prep with Ribo-Zero Plus effectively depletes rRNA levels for human, mouse, rat, and bacterial species in a single-tube reaction. Results are compared to TruSeq Stranded Total RNA paired with Ribo-Zero Gold for mammalian species and Ribo-Zero Bacteria for E. coli (B. subtilis data not shown). Bottom- Globin mRNA depletion from human peripheral blood leukocytes with Illumina Stranded Total RNA Prep with Ribo-Zero Plus
Illumina Stranded Total RNA Prep provides high coverage uniformity for (A) high-quality and synthetically degraded UHR RNA (RIN=2) and (B) FFPE RNA at input levels of 100 ng and 10 ng. The FFPE sample had a DV200 quality score of 55%. All libraries were sequenced on a NovaSeq 6000 System at 50M reads. Data analysis was performed using the BaseSpace RNA-Seq Alignment App v2.0.1.
Top- Comparison of performance metrics — Illumina Stranded Total RNA Prep with Ribo-Zero Plus was compared against TruSeq Stranded Total RNA with Ribo-Zero. Illumina Stranded Total RNA Prep with Ribo-Zero Plus was more effective, particularly at low inputs of 10 ng and 1 ng total UHR RNA. Libraries were sequenced on a NextSeq 550 System, subsampled to 30M reads. Percent duplicates were calculated by subsampling to 4M reads and analyzed using the BaseSpace RNA-Seq Alignment App v2.0. Bottom- Performance metrics for Illumina Stranded Total RNA Prep with Ribo-Zero Plus
Illumina Stranded Total RNA Prep achieves high data concordance between (A) input amounts of 1 ng and 100 ng UHR RNA and (B) technical replicates of 10 ng FFPE RNA. Libraries were sequenced on a NovaSeq 6000 System at 2 × 74 bp. Data analysis was performed using the BaseSpace RNA-Seq Alignment App v2.0.1.
Illumina Stranded Total RNA Prep delivers a fast workflow with reduced handson time. Times may vary depending on equipment used, number of samples processed, automation procedures, or user experience.