Nextera XT DNA文库制备试剂盒
支持低DNA起始量，仅需90分钟，即可为小型基因组、PCR扩增子、质粒或cDNA制备好测序文库。Read More...
Library Prep
Illumina Advantage Products
Index Adapters
借助 Nextera 技术，DNA 在单管酶促反应中同时被片段化并用测序接头进行标记。 Nextera XT 支持仅 1 ng 的超低 DNA 输入量。它支持广泛的输入样本，包括小基因组、大于 300 bp 的 PCR 扩增子、质粒、微生物基因组、串联扩增子和双链 cDNA。
每个 Nextera XT 库可复用多达 384 个样本，可用于需要更高通量的项目。此外，该试剂盒包括创新的基于微珠的样品标准化，无需在合并和测序之前对文库进行量化。使用 Nextera XT 试剂盒制备的文库与所有 Illumina 测序仪兼容。
用于 Illumina-Nextera DNA UD 索引集 A、B、C 和 D 索引的 IDT 提供多达 384 个独特的双索引，允许准确分配读取和高效使用流动槽。这些独特的双索引代码使用 10 bp 代码。碱基对索引代码的这种变化需要对测序运行设置进行调整。
该产品也可作为 Illumina Advantage (TG) 产品。Illumina Advantage大规模测序产品具有特定批次出货和测试、延长货架寿命和先进的变更通知，以提高实验室效率。
For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.
Read Tech Note
| 仪器 | 推荐的样本数 | 读长 |
|---|---|---|
| MiSeq System | Up to 384 samples per run | Up to 2 x 300 bp |
| NextSeq 550 System | Up to 384 samples per run | Up to 2 x 150 bp |
| Nextera XT DNA文库制备试剂盒 | Illumina DNA Prep | |
|---|---|---|
| Assay Time | ~5.5 hours from DNA extraction to normalized library. (Library prep time: ~90 minutes). | ~3-4 hours (from DNA extraction to normalized library) |
| Description | Fast library prep optimized for research on small genomes, PCR amplicons, and plasmids. | A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more. |
| Hands-On Time | 15 minutes | 1-1.5 hours |
| Input Quantity | 1 ng DNA | Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). |
| Mechanism of Action | Enzymatic fragmentation | 连接磁珠的转座酶 |
| Method | 16s rRNA Sequencing, Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing | Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing |
| Multiplexing | Up to 384 uniquely indexed samples may be pooled and sequenced together. | Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations |
| Specialized Sample Types | Low-Input Samples, Single Cells, 不兼容FFPE | Blood, Not FFPE-Validated, Saliva |
| Species Details | Compatible with any species | Compatible with any species |
| Target Insert Size | 300 bp–1.5 kb | ~350bp |
Single-cell mRNA-Seq with the MiSeq and HiSeq Systems is enabling Allen Institute researchers to classify V1 neural cells.
Read MoreGenomic studies with the HiSeq and MiSeq Systems are enabling researchers to track Ebola outbreaks and understand the impact of the virus’s rapid mutation rate.
Read More
The combination of Nextera XT and rapid sequencing with the MiSeq System provides a comprehensive DNA to data workflow in only 8 hours.
The Nextera XT Library Preparation Kit eliminates the need for library quantification before sample pooling and sequencing. Libraries of equivalent concentrations are created by employing bead-based sample normalization, as simple as pipetting 5 μl of each library to be sequenced.
Nextera Library Validation and Cluster Density Optimization
Technical Note | PDF< 1 MB
Technical Note | PDF< 1 MB
Nextera XT Library Prep: Tips and Troubleshooting
Technical Note | PDF< 1 MB
Sequencing the Rapidly Evolving Influenza A Virus on the MiSeq System
Application Note | PDF< 1 MB
Nextera XT DNA Library Preparation Kit
Data Sheet | PDF< 1 MB
Nextera XT DNA Library Prep Kit Documentation
Nextera XT DNA Library Prep Kit Consumables & Equipment List Documentation
Index Adapters Pooling Guide Documentation
Nextera XT DNA Library Prep Kit Checklist Documentation
Illumina Experiment Manager User Guide Documentation
Nextera XT DNA Library Prep Kit Reference Guide Documentation
自定义协议选择器
Generates customized, end-to-end instructions
