Illumina DNA Prep

一个快速、集成的工作流程,适合广泛的应用,从人类全基因组测序到扩增子、质粒和各种微生物。Read More...
选择产品
What products do I need?
Library Prep

Illumina® DNA Prep, (M) Tagmentation (24 Samples)

20018704

Illumina® DNA Prep, (M) Tagmentation (96 Samples)

20018705


Index Adapters

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

Nextera™ DNA CD Indexes (24 Indexes, 24 Samples)

20018707

Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)

20018708


Services

Illumina DNA Prep Training

20022900

配件产品
What accessories do I need?

Flex Lysis Reagent Kit (96 reactions)

20018706

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

NextSeq PhiX Control Kit

FC-110-3002

Product Highlights

Illumina DNA Prep为多个全基因组测序应用带来灵活性。

  • 最快的Illumina文库制备流程,总时间约为3.5小时
  • 高度灵活,适应样本类型、DNA起始量和应用的差异
  • 文库制备性能经过优化,产生值得信赖的结果

Learn more about the technology

节省时间和资源

Illumina DNA Prep采用一个快速且用户友好的工作流程。微珠tagmentation技术将文库制备的总时间(从DNA提取到文库归一化)缩短至3.5小时。

简化实验室操作

Illumina DNA Prepx的工作流程支持广泛的DNA起始量(1-500 ng),多种类型的样本,以及小型和大型基因组。这个流程包括从血液、唾液、干血斑*或细菌菌落*中提取DNA。灵活地对人类或其他复杂的大型基因组以及扩增子或微生物群落进行测序,这一切通过单个试剂盒都能实现。

Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

获得可靠的结果

在满足不同研究需求的同时,Nextera DNA Flex的工作流程带来大小一致的插入片段、均一的覆盖和优化的性能,不论DNA起始量或基因组大小如何。这种基于微珠的技术最大限度地减少了偏向性和错误机会,带来高度可重现的测序数据。

获得灵活的通量选项

IDT® for Illumina®-Nextera™ DNA UD Indexes set A、B、C和D标签提供多达384个唯一双标签,可准确分配read,高效利用流动槽。 这些唯一双标签码使用10个碱基对码。碱基对标签码的这种改变需要调整测序运行设置。

Nextera DNA CD Indexes支持多达96个样本的组合双标签。24规格的CD标签以试管的形式提供,96规格的CD标签以孔板的形式提供

Related Notification

For whole-genome sequencing, Illumina DNA Prep is the recommended replacement for the Nextera DNA Library Prep Kit, which has been discontinued. If you were using Nextera DNA (Cat. No. FC-121-1030) or stand-alone components (Cat. Nos. 15027865 and 15027866) for ATAC-Seq** or other custom applications, the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit is our suggested alternative.

*Demonstrated protocols available.

**Customer-reported application

探索文库制备的新时代

通过这种互动体验,感受Nextera技术为您的实验室带来的力量。Nextera文库制备试剂盒解决方案支持各种应用中的全基因组测序、灵活兼容Illumina测序仪,整合血液和唾液的样本制备,且无需定量。

查看信息图
Nextera DNA Flex Library Prep Kit简介

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
NextSeq 550 System 1 sample per run (high output; based on 30× coverage of a human genome) Up to 2 × 150 bp
NovaSeq 6000 System 2–10 samples per run  (dual flow cell; based on 30× coverage of a human genome) Up to 2 × 125 bp (rapid run) 
Up to 2 × 150 bp (high output)

产品比较

Illumina DNA Prep TruSeq DNA PCR-Free TruSeq DNA Nano
Assay Time ~3-4 hours (from DNA extraction to normalized library) 5 hours total assay time ~6 hours total assay time
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Hands-On Time 1-1.5 hours 4 hours ~4 hours
Input Quantity Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). 1 ug DNA 100 ng genomic DNA
Mechanism of Action Bead-linked transposome Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free.
Method Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing Genotyping by Sequencing , Shotgun Sequencing , Whole-Genome Sequencing Genotyping by Sequencing , Shotgun Sequencing , Whole-Genome Sequencing
Multiplexing Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon)
Species Category Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish Human, Mammalian, Mouse, Other, Plant, Rat Human, Mammalian, Mouse, Other, Plant, Rat

方法特异的工作流程示例

 

支持数据和图像

工作原理

标题:The process begins with unfragmented DNA binding to the BLT. Since each transposome is only able to perform the reaction once, saturation of the BLT acts as a DNA normalization step, saving time from doing it at the end of the library prep. It also gives more even and uniform coverage across the region with little region-specific bias. Note that at least 100 ng of input DNA is required for integrated normalization

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