AmpliSeq for Illumina Focus Panel

用于研究52个与实体瘤相关的基因的靶向DNA和RNA研究panel。Read More...
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Panel

AmpliSeq™ Focus Panel for Illumina®

20019164

Ampliseq™ for Illumina® Focus Panel MiSeq Kit

20024183


Library Prep

AmpliSeq™ Library PLUS for Illumina®(24次反应)

20019101

AmpliSeq™ Library PLUS for Illumina®(96次反应)

20019102

AmpliSeq™ Library PLUS for Illumina®(384次反应)

20019103


Index Adapters

AmpliSeq™ UD Indexes for Illumina®(24标签,24样本)

20019104

AmpliSeq™ CD Indexes Set A for Illumina®

20019105

AmpliSeq™ CD Indexes Set B for Illumina®

20019106

AmpliSeq™ CD Indexes Set C for Illumina®

20019107

AmpliSeq™ CD Indexes Set D for Illumina®

20019167

AmpliSeq™ CD Indexes Set A-D for Illumina®(384标签,384样本)

20031676

配件产品

Ampliseq™ cDNA Synthesis for Illumina®

20022654

AmpliSeq™ for Illumina® Sample ID Panel

20019162

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

AmpliSeq™ Library Equalizer for Illumina®

20019171

Product Highlights

AmpliSeq for Illumina Focus Panel是一种靶向重测序分析套装,用于对52种与实体瘤具有已知关联的基因的生物标记物进行分析。利用这种Focus Panel,研究人员能够同时分析DNA和RNA。主要特点包括:

相关基因内容
  • 52个与实体瘤相关基因的生物标记
快速的一体化工作流程
  • 可在一天内使用起始量低至1 ng的高质量样本或10 ng福尔马林固定、石蜡包埋(FFPE)的样本制备直接测序的文库
准确的数据
  • 使用本地或云端分析方法检测频率低至5%的体细胞突变

Focus Panel是集成工作流程的一部分,该工作流程包括基于AmpliSeq for Illumina聚合酶链式反应(PCR)的文库制备、采用Illumina边合成边测序(SBS)的新一代测序(NGS)技术以及自动化分析过程。

即用型panel助您节省鉴定目标基因、设计引物和优化panel所需的时间和精力。

深入了解AmpliSeq for Illumina

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
iSeq 100 System 8 samples per run (assumes minimum coverage of 500×) 2 × 150 bp
MiniSeq System Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System Samples per run (by reagent kit version): v2 micro: 8, v2: 30, v3: 48 (assumes minimum coverage of 500×) 2 × 150 bp

产品比较

AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Comprehensive Panel v3 AmpliSeq for Illumina Cancer Hotspot Panel v2 TruSight Tumor 15
Assay Time 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer Type Solid Tumor Solid Tumor Solid Tumor, 泛癌种 Solid Tumor
Content Specifications DNA and RNA targets for 52 oncogenes DNA and RNA targets for 161 oncogenes Hotspot regions of 50 genes with known associations to cancer
Description Somatic analysis research on 52 genes associated with solid tumor cancers. Somatic analysis research on hotspot and full-length targets of genes associated with solid tumor cancers. Somatic analysis research into hotspot regions of 50 cancer-related genes.
Hands-On Time < 1.5 hours < 1.5 hours < 1.5 hours
Input Quantity 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool)
Method Amplicon Sequencing , 靶向DNA测序 , 靶向RNA测序 Amplicon Sequencing , 靶向DNA测序 , 靶向RNA测序 Amplicon Sequencing , 靶向DNA测序 Amplicon Sequencing , 靶向DNA测序
Multiplexing 96 dual index combinations 96 dual index combinations 96 dual index combinations
Nucleic Acid Type DNA, RNA DNA, RNA DNA DNA
Specialized Sample Types FFPE Tissue Blood, FFPE Tissue Blood, FFPE Tissue FFPE Tissue
Species Category 人类 人类 人类 人类

具体的方法工作流程范例

 

支持数据和图像

 

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