AmpliSeq for Illumina Cancer Hotspot Panel v2

Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.Read More...
选择产品
What products do I need?
Panel

AmpliSeq™ Cancer HotSpot Panel v2 for Illumina®

20019161


Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103


Index Adapters

AmpliSeq™ CD Indexes Set A for Illumina® (96 Indexes, 96 Samples)

20019105

配件产品

AmpliSeq™ for Illumina® Sample ID Panel

20019162

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

AmpliSeq™ Library Equalizer for Illumina®

20019171

Product Highlights

The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database. Key features include:

Relevant Gene Content
  • Target ~2800 COSMIC mutations from 50 oncogenes and tumor suppressor genes
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) samples
Accurate Data
  • Detect somatic mutations down to 5% variant allele frequency using local or cloud-based analysis

The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

Frequently Purchased Together

规格

计划推荐

仪器 推荐的样本数 读长
iSeq 100 System 每次运行16个样本(假设最小覆盖度为500倍) 2 × 150 bp
MiniSeq System 每次运行的样本:中通量:32,高通量:96(假设最小覆盖度为500倍) 2 × 150 bp
MiSeq System 每次运行的样本(按试剂盒版本):v2 nano:4,v2 micro:16,v2:60,v3:96(假设最小覆盖度为500倍) 2 × 150 bp

Method-Specific Workflow Example

 

Related Products

AmpliSeq for Illumina Focus Panel

用于研究52个与实体瘤相关的基因的靶向DNA和RNA研究panel。


TruSight Tumor 15

这款靶向测序panel可在单次检测中以简便快速的工作流程评估实体瘤中的15个常见突变基因。


AmpliSeq for Illumina Custom DNA Panel

专为特异性靶点或感兴趣的基因组内容测序进行优化的靶向定制研究panel。


References
  1. Kingsmore S. Catalogue of Somatic Mutations in Cancer – Home Page. http://cancer.sanger.ac.uk/cosmic. Accessed October 25, 2017.