AmpliSeq for Illumina BRCA Panel

用于研究BRCA1BRCA2基因的体细胞和生殖系变异的靶向研究panel。Read More...
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Panel

AmpliSeq™ BRCA Panel for Illumina®

20019168


Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103


Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

AmpliSeq™ CD Indexes Set A for Illumina®

20019105

AmpliSeq™ CD Indexes Set B for Illumina®

20019106

AmpliSeq™ CD Indexes Set C for Illumina®

20019107

AmpliSeq™ CD Indexes Set D for Illumina®

20019167

AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

配件产品

AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

AmpliSeq™ Library Equalizer for Illumina®

20019171

Product Highlights

The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes. Key features include:

Relevant Gene Content
  • Target all exonic regions and flanking intronic sequences of BRCA1 and BRCA2
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
  • Detect somatic mutations as low as 5% variant allele frequency using local or cloud based analysis

The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

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规格

计划推荐

仪器 推荐的样本数 读长
iSeq 100 System 12 samples per run (assumes minimum coverage of 500×)
96 samples per run (assumes minimum coverage of 50x)
2 × 150 bp
2 × 150 bp
MiniSeq System Samples per run: mid output: 24, high output: 80 (assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System Samples per run at 500x minimum coverage (by reagent kit version): v2 nano: 3, v2 micro: 12, v2: 48, v3: 80
Samples per run at 50x minimum coverage (by reagent kit version): v2 nano: 32, v2 micro: 96
2 × 150 bp
2 × 150 bp

产品比较

AmpliSeq for Illumina BRCA Panel TruSight Cancer癌症相关基因探针集
Assay Time 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 1.5 days
Cancer Type Solid Tumor 泛癌种
Content Specifications Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers.
Description Germline and somatic analysis studies of BRCA1 and BRCA2 . Germline mutation detection research for common and rare cancers.
Hands-On Time < 1.5 hours 5 hours
Input Quantity 1–100 ng (10 ng recommended per pool) 50 ng DNA
Method Amplicon Sequencing , 靶向DNA测序 靶向DNA测序 , 靶向富集
Multiplexing 96 dual index combinations Up to 96-plex
Specialized Sample Types Blood, FFPE Tissue Not FFPE-Compatible
Variant Class 体细胞变异, 单核苷酸多态性 (SNPs), 插入缺失 (indels), 生殖系变异 生殖系变异

Method-Specific Workflow Example

 

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