Illumina DRAGEN Bio-IT Platform

llumina DRAGEN(Dynamic Read Analysis for GENomics)Bio-IT平台能够对新一代测序(NGS)数据进行非常快速的二级分析。Read More...
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DRAGEN CS Lvl 1 1 year License

20027361

DRAGEN CS Lvl 2 1 year License

20027362

DRAGEN CS Lvl 3 1 year License

20027363

DRAGEN CS Lvl 4 1 year License

20027364

DRAGEN CS Lvl 5 1 year License

20027365

Illumina DRAGEN Server v3

20040619

产品特色

The Illumina DRAGEN Bio-IT Platform offers:
  • Accurate data: Detects small variants with high analytical sensitivity and specificity1
  • Lossless genomic compression: Reduces FASTQ file sizes up to 5X with DRAGEN ORA (Original Read Archive) lossless compression
  • Robust speed: Processes an entire human genome at 30× coverage in about 25 minutes2,3
  • Frequent releases: Includes releases of regular enhancements and upgrades
  • Various applications: Analyzes next-generation sequencing (NGS) data from whole genomes, exomes, and RNA

The Illumina DRAGEN Bio-IT platform and data submission apps on BaseSpace Sequence Hub also support the SARS-CoV-2 Data Toolkit. Learn More.

DRAGEN Options

DRAGEN on BaseSpace Sequence Hub

DRAGEN on Illumina Connected Analytics

DRAGEN On-Premise

DRAGEN on NextSeq 1000/2000

超快速的分析

DRAGEN平台可以在25分钟之内以30倍的覆盖度在本地处理整个人类基因组的NGS数据,而传统的基于CPU的系统需要的时间超过15小时。其创造了基因组数据分析的两项世界速度记录。2,3

经济高效的解决方案

When run on Illumina's BaseSpace Sequence Hub or Illumina Connected Analytics, analysis for a 30x genome is ~$5/sample and an exome at 100x coverage ~$3/sample.

高度精确的结果

In the 2020 PrecisionFDA Truth Challenge V2, DRAGEN won Difficult to Map Regions and All Benchmark Regions on Illumina Sequencing data.1

多样化的应用

DRAGEN平台可分析全基因组、全外显子组、靶向panel、生殖系和体细胞数据集以及RNA测序实验的测序数据。

Lossless Genomic Compression

DRAGEN ORA (Original Read Archive) compression technology is optimized for high compression ratios of FASTQ files, as well as rapid compression and decompression, all while preserving data integrity.

经常更新

经常更新,不断扩展并改进DRAGEN平台,能适应新的应用并满足不断发展的行业标准。DRAGEN的更新可通过版本来管理,用户可以根据需要选择加入或退出新的更新。

DRAGEN在PrecisionFDA Truth Challenge V2中赢得了关键奖项

Illumina DRAGEN团队非常高兴地宣布,他们在Illumina测序数据的难绘制区域和所有基准区域中展现了优于其他技术的性能。这标志着优化主要新功能的开始,包括结构变异、拷贝数变异和重复延长。我们的成功证明,我们现在能以更高的准确性检出难绘制区域中的变异。

了解更多

DRAGEN平台使各种规模和学科的实验室能够利用其基因组数据挖掘更多的信息。DRAGEN平台使用高度可重新配置的现场可编程门阵列技术(FPGA),通过加速硬件来实施基因组分析算法,例如BCL转换、定位、比对、分选、重复标记和单倍型变异检出。DRAGEN平台的基本功能解决了基因组分析中的常见难题,例如计算时间长和数据量大。

DRAGEN平台可在不影响精确度的前提下,实现快速、灵活和经济高效。DRAGEN平台的可重编程特性使Illumina能够开发自定义算法,还允许进行改进以便适于未来的应用。

关键DRAGEN应用

  DRAGEN服务器 BaseSpace Illumina Connected Analytics NextSeq 1000/2000
样本拆分(BCL转换)        
DRAGEN ORA lossless genomic compression        
定位和比对        
全基因组(生殖系和体细胞)       Germline only
外显子组富集(生殖系和体细胞)        
COVIDSeq        
RNA Pathogen Detection        
RNA-Seq(基因融合和定量)        
Single-Cell RNA        
Joint Genotyping        
甲基化        
TSO 500 Liquid        
Metagenomics        

DRAGEN还可以通过AWS MarketPlace上的Amazon机器映像(AMI)运行

Pipeline Description Variant Types Detected Metrics Provided
DRAGEN Demultiplexing Rapid demultiplexing of NGS analysis N/A N/A
DRAGEN Compression DRAGEN ORA compression is optimized for high compression ratios of FASTQ files, as well as rapid compression and decompression, all while preserving data integrity. N/A
  • Compression Ratio
  • Run Time
DRAGEN Map + Align The DRAGEN Map + Align can be run as a standalone or as part of DRAGEN’s suite of pipelines N/A
  • Mapping metrics
  • Duration Metrics
  • Coverage Metrics
DRAGEN Germline The DRAGEN Germline Pipeline provides end-to-end NGS analysis, including advanced error model calibration for increased accuracy, and repeat expansion detection and genotyping through Illumina Expansion Hunter.
  • SNV
  • CNV
  • SV
  • Repeat Expansions
  • Mapping metrics
  • Duration Metrics
  • Coverage Metrics
  • Variant Metrics
  • Callability Report
DRAGEN Somatic The DRAGEN Somatic Pipeline includes tumor-only and tumor–normal modes, designed for detecting somatic variants in tumor samples. Both modes make no ploidy assumptions, enabling detection of low-frequency alleles.
  • SNV
  • CNV
  • SV
  • TMB
  • MSI
  • HLA
  • Mapping metrics
  • Duration Metrics
  • Coverage Metrics
  • Variant Metrics
  • Callability Report
DRAGEN Enrichment The DRAGEN Enrichment Pipeline combines DRAGEN’s germline and somatic callers into a pipeline designed specifically for analyzing enrichment samples. Includes a full suite of enrichment metrics and reporting.
  • SNV
  • CNV
  • SV
  • Mapping metrics
  • Duration Metrics
  • Coverage Metrics
  • Variant Metrics
  • Callability Report
DRAGEN RNA The DRAGEN RNA Pipeline performs transcriptome analysis starting with splice junction discovery and alignment, followed by rapid alignment and splice junction mapping and quantification. For differential expression, Illumina recommends the DRAGEN Differential Expression app on BaseSpace Sequence Hub.
  • Gene fusion
  • Mapping metrics
  • Duration Metrics
  • Coverage Metrics
  • Variant Metrics
  • Callability Report
DRAGEN Single Cell RNA The DRAGEN Single Cell RNA pipeline performs demultiplexing, cell-barcode and UMI error correction, sequence alignment, and quantification of gene expression. N/A
  • Mapping Metrics
  • Duration Metrics
  • Coverage Metrics
  • Callability Report
  • Cell Metrics
DRAGEN Joint Genotyping The DRAGEN Joint Genotyping/Population Pipeline calls variants jointly across multiple genomes and scales to large cohorts of samples at expedited speeds with uncompromising accuracy.
  • SNV
  • CNV
  • SV
  • Repeat Expansions
  • Mapping metrics
  • Duration Metrics
  • Coverage Metrics
  • Variant Metrics
  • Callability Report
DRAGEN Methylation The DRAGEN Methylation Pipeline performs alignment, methyl calling, and calculates alignment and methylation metrics. N/A
  • Mapping metrics
  • Duration Metrics
  • Coverage Metrics
  • Variant Metrics
  • Callability Report
DRAGEN Reference Builder Accepts FASTA files, and builds the proprietary reference used by the DRAGEN apps. N/A N/A
DRAGEN TruSight Oncology 500 ctDNA Analysis Software Secondary analysis support for Illumina’s TruSight Oncology 500 ctDNA. Only available on the local DRAGEN Server (version 3)
  • SNV
  • CNV
  • DNA fusions
  • MSI
  • TMB
  • Mapping metrics
  • Duration Metrics
  • Coverage Metrics
  • Variant Metrics
  • Callability Report

Phosphorus的联合创始人兼首席执行官Alexander Bisignano介绍了该公司如何使用DRAGEN Bio-IT平台以可承受的价格在现场进行基因组数据分析。

阅读访谈录
SARS-CoV-2 NGS数据工具包

llumina通过提供免费的集成式软件工具包,帮助研究人员更轻松地检测和识别SARS-CoV-2,并将他们的发现贡献给社区。

了解更多
SARS-CoV-2 NGS Data Toolkit

BaseSpace Sequence Hub上的DRAGEN结合了DRAGEN平台的速度、准确性与BaseSpace Sequence Hub的安全性、经济和直观界面。

  • 适用于不同专业水平的按键式分析
  • 直接从仪器中传输数据并进行快速分析
  • 低成本,没有硬件投入
  • 利用BaseSpace Sequence Hub和Amazon Web Services(AWS)的安全性、合规性和存储功能

深入了解BaseSpace Sequence Hub

深入了解Illumina Connected Analytics

DRAGEN on BaseSpace Sequence Hub
 

与传统的基于CPU的系统相比,本地DRAGEN可以在短时间内进行二级分析,同时保持高精确度。

  • 在本地分析和储存数据
  • 支持不同水平的命令行界面
  • 可代替多达30个传统计算实例
  • 在25分钟内以30倍的覆盖度处理整个人类基因组的NGS数据
  • 一个装置可支持两个满负荷运行的NovaSeq系统

阅读数据表

DRAGEN On-Premise
 

机载DRAGEN Bio-IT平台可提供准确的超快速二级分析方案。

  • 多种DRAGEN生信分析工具可供选择
  • 两小时内就能生成结果
  • 提供优化的硬件加速算法,可用于各种基因组分析解决方案,包括BCL转换、基因组定位、序列比对、序列排序、重复标记和变异检出
  • 使用同类优秀的流程算法可减少对外部生信专家的依赖

阅读数据表

View Video
Broad研究所

Broad研究所的Anthony Philippakis和Illumina的Susan Tousi是合作伙伴,他们就GATK与Illumina测序仪的整合以及DRAGEN平台的实施如何促进硬件加速和数据分析标准化进行了讨论。

观看视频
 

GeneDx利用DRAGEN从外显子组扩展到基因组

借助DRAGEN,GeneDx现在能够在保持低成本、缩短周转时间和提高准确性的同时增长业务。

阅读文章
Rady儿童医院

该医院创造了最快基因诊断(19.5小时)的吉尼斯世界纪录。DRAGEN平台显著提高了测序后进行基因组数据定位和比对的速度。1

阅读文章
Xing Cancer Care

了解Basespace Sequence Hub和DRAGEN如何简化该实验室的NGS数据分析。

观看视频

阅读文章
群体测序

快速分析测序基因组;随着计算工具的不断改进,加速重分析。

NICU和遗传病

在快速获得结果至关重要时,减少基因组分析所需的时间。

癌症研究

准确、快速地分析仅肿瘤和肿瘤/正常样本。

农业基因组学

利用所提供的参考资料分析具有不同基因组复杂性的动植物。

临床和转化研究

以快速的周转时间和高精确度分析靶向外显子组数据,确保重现性和高质量的结果。

参考文献
  1. PrecisionFDA Truth Challenge V2: Calling Variants from Short and Long Reads in Difficult-to-Map Regions. precision.fda.gov/challenges/10. Accessed November 3, 2020.
  2. Bio IT World. Children’s Hospital Of Philadelphia, Edico Set World Record For Secondary Analysis Speed. October 23, 2017. www.bio-itworld.com/2017/10/23/childrens-hospital-of-philadelphia-edico-set-world-record-for-secondary-analysis-speed.aspx. Accessed September 19, 2018.
  3. The San Diego Union Tribune. Rady Children's Institute sets Guinness world record. February 12, 2018. www.sandiegouniontribune.com/news/health/sd-no-rady-record-20180209-story.html. Accessed September 19, 2018.

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