Illumina has partnered with CareDx to distribute and support the TruSight HLA v2 Sequencing Panel and TruSight HLA Assign software. For TruSight HLA ordering and technical support, visit the CareDx TruSight HLA webpages.

TruSight HLA v2 Sequencing Panel

Unambiguous, phase-resolved HLA typing in a single assay using proven Illumina NGS technology and Assign analysis software.Read More...
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TruSight HLA v2 Sequencing Panel (24 samples)

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TruSight HLA v2 Sequencing Panel (24 samples, automated)

20005170

产品特色

The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

  • High-resolution sequencing of 11 HLA loci
  • Workflow with library preparation, sequencing and data analysis/reporting
  • Accurate and high-resolution HLA typing for unambiguous results
Capture Full HLA Gene Sequences

TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis

The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexible options for post-analysis reporting of results.

High-Accuracy HLA Typing

The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works

TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights

The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Frequently Purchased Together

规格

Method-Specific Workflow Example

 

Supporting Data and Figures

 
TruSight HLA v2 Types 11 HLA Loci
Loci Target Sequence
HLA-A 4.1 kb (entire gene)
HLA-B 2.8 kb (exon 1 - intron 6)
HLA-C 4.2 kb (entire gene)
HLA-DRB1/3/4/5 4.6 - 5.1 kb (exon 2 - intron 4)
HLA-DQB1 6.9 kb (entire gene)
HLA-DPB1 9.7 kb (exon 2-3' UTR)
HLA-DQA1 7.3 kb (entire gene)
HLA-DPA1 10.3 kb (entire gene)

With the introduction of the TruSight HLA v2 Sequencing Panel, which has been optimized to improve accuracy, increase efficiency, and reduce cost, the TruSight HLA v1 Sequencing Panel has been discontinued. Illumina remains committed to providing you with high-quality support and service during this transition.

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