NextSeq 550 System High-Output Kit* | NextSeq 550 System Mid-Output Kit* | ||||
---|---|---|---|---|---|
Read Length | Total Time† | Output | Read Length | Total Time† | Output |
2 × 150 bp | 29 hrs | 100–120 Gb | 2 × 150 bp | 26 hrs | 32.5–39 Gb |
2 × 75 bp | 18 hrs | 50–60 Gb | 2 × 75 bp | 15 hrs | 16.25–19.5 Gb |
1 × 75 bp | 11 hrs | 25–30 Gb |
*Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All NextSeq 550 kits are paired-end capable.
†Total time includes cluster generation, sequencing, and base calling on a NextSeq 550 System enabled with dual-surface scanning.
NextSeq 550 System High-Output Kit | NextSeq 550 System Mid-Output Kit | |
---|---|---|
Single Reads | Up to 400 Million | Up to 130 Million |
Paired-End Reads | Up to 800 Million | Up to 260 Million |
NextSeq 550 System High-Output Kit | NextSeq 550 System Mid-Output Kit |
---|---|
> 75% bases higher than Q30 at 2 × 150 bp | > 75% bases higher than Q30 at 2 × 150 bp |
> 80% bases higher than Q30 at 2 × 75 bp | > 80% bases higher than Q30 at 2 × 75 bp |
> 80% bases higher than Q30 at 1 × 75 bp |
††A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
NextSeq 550 System High-Output Kit | NextSeq 550 System Mid-Output Kit |
---|---|
1 human whole genome | 3 exomes |
12 exomes | 12 enrichment panels |
16 transcriptomes | 96 amplicon panels |
Array | Scan Time Per Array | Scan Time Per Sample |
---|---|---|
Infinium MethylationEPIC BeadChip | 40 minutes | 5 minutes |
Infinium CytoSNP-850K BeadChip | 40 minutes | 5 minutes |
HumanCytoSNP-12 BeadChip | 40 minutes | 3.3 minutes |
Infinium HumanKaryomap-12 BeadChip | 40 minutes | 3.3 minutes |
Fast, flexible, high-throughput benchtop sequencers enable whole-genome, transcriptome, and targeted resequencing applications with the accuracy of Illumina SBS technology.
View Specification SheetThe NextSeq 550 System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
View SBS videoThe NextSeq 550 System leverages 2-channel SBS chemistry to offer efficient sequencing and data generation. 2-channel SBS reduces cycle and data processing times, while delivering the same high quality and accuracy that sets Illumina systems apart. This innovation makes NGS an affordable solution for use as an everyday tool.
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