Big advantages in a little package
Highly accurate data, fast run times, and a maximum output of 1.2 Gb in a simple, compact benchtop sequencing system
What is read length?
Sequence read length refers to the length of each sequenced DNA fragment and whether it is sequenced from one end or both ends. For example, a 2 × 150 bp run generates two reads (forward and reverse) of 150 base pairs for each DNA fragment. Longer read lengths are preferred over shorter read lengths because they have a better probability of spanning repeats in the DNA sequence.
| Read length | iSeq 100 i1 Reagent v2 |
|---|---|
| 1 × 36 bp | 144 Mb |
| 1 × 50 bp | 200 Mb |
| 1 × 75 bp | 300 Mb |
| 2 × 75 bp | 600 Mb |
| 2 × 150 bp | 1.2 Gb |
a. Specifications based on Illumina PhiX control library at supported cluster densities (174–200 k/mm2 clusters passing filter).
| Application | iSeq 100 i1 Reagent v2b |
|---|---|
Small genome sequencing per run 5–10 Mb genomes, 30× coverage, 2 × 150 bp |
1–8 |
Targeted gene expression profiling per run Up to 500 targets, 1 × 50 bp |
1–48 |
Targeted amplicon sequencing per run Up to 3000 amplicons, 2 × 150 bp |
1–48 |
a. All sample throughputs are estimates and are based on single flow cell runs.
b. Number of samples per run using the iSeq 100 i1 Reagent v2.
What are reads passing filter?
The percentage of clusters passing chastity filtering, with each passing filter cluster providing a read (single-end reads) or two reads (paired-end reads). Learn more about reads passing filter.
| iSeq 100 i1 Reagent v2 | |
|---|---|
| Single-end reads | 4M |
| Paired-end reads | 8M |
a. Specifications based on Illumina PhiX control library at supported cluster densities (174–200 k/mm2 clusters passing filter).
What is a quality score?
A quality score, or Q-score, is a prediction of the probability of an error in base calling. Higher Q-scores indicate a smaller probability of error. A quality score of 30 represents an error rate of one in 1000, with a corresponding call accuracy of 99.9%.
| Read length | iSeq 100 i1 Reagent v2 |
|---|---|
| 1 × 36 bp | > 85% of bases higher than Q30 |
| 1 × 50 bp | > 85% of bases higher than Q30 |
| 1 × 75 bp | > 80% of bases higher than Q30 |
| 2 × 75 bp | > 80% of bases higher than Q30 |
| 2 × 150 bp | > 80% of bases higher than Q30 |
a. The percentage of bases > Q30 is averaged across the entire run.
| Read length | iSeq 100 i1 Reagent v2 |
|---|---|
| 1 × 36 bp | 9.5 hr |
| 1 × 50 bp | 10 hr |
| 1 × 75 bp | 11 hr |
| 2 × 75 bp | 14 hr |
| 2 × 150 bp | 19 hr |
a. Total time includes cluster generation, sequencing, and base calling on an iSeq 100 System.
iSeq 100 instrument specifications
Sequencing by synthesis chemistry
The iSeq 100 System harnesses proven Illumina sequencing by synthesis (SBS) chemistry to deliver highly accurate data and robust performance for a broad range of applications. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.
The iSeq 100 System combines Illumina SBS chemistry with complementary metal-oxide semiconductor (CMOS) technology to deliver one-channel SBS chemistry. One-channel SBS chemistry on a CMOS chip helps to reduce instrument cost and allows for a smaller benchtop footprint, all while delivering accurate data.
Sequencing quality scores are a measure of the uncertainty of base calls, or the probability of a base call being wrong. Understand quality scores and what they mean for your sequencing run.
Sequencing coverage requirements vary by application. Learn how to estimate and achieve your desired next-generation sequencing (NGS) coverage level.
Paired-end vs single-read sequencing
Paired-end runs sequence both DNA ends, for easier analysis of rearrangements, novel transcripts, and more. Single-end runs offer an economical alternative. Learn more about the key differences between these sequencing read types.