Robust methylation profiling microarray providing extensive coverage of CpG islands, genes, and enhancers for epigenome-wide association studies.
Sample throughput
Number of samples
Number of markers
The Infinium MethylationEPIC v2.0 kit is a genome-wide methylation screening tool targeting > 935K CpG sites in the most biologically significant regions of the human methylome.
A comparatively low total cost per sample and scalability enables labs to screen large cohorts efficiently.
Illumina methylation BeadChips have powered > 10 years of groundbreaking research, with most published epigenome-wide association studies (EWAS) to date completed on MethylationEPIC or its predecessor. Harness methylation data in data repositories like the Gene Expression Omnibus (GEO) or The Cancer Genome Atlas (TCGA).
The assay is FFPE-compatible, enabling studies to be completed on large biorepositories of tumors. It is also compatible with fresh/frozen tissue, whole blood, buccal cells, cfDNA, saliva, and other sample types.
| Assay type | Infinium HD Methylation |
|---|---|
| Automation capability | Automated Array Loader |
| Description |
The Infinium MethylationEPIC v2.0 BeadChip is a genome-wide methylation screening tool with enhanced, expert-selected functional content. The kit is processed on the iScan or NextSeq 550 Systems and follows a straightforward, user-friendly workflow that does not require sample pooling and indexing. |
| Input quantity | 250 ng DNA |
| Instruments | NextSeq 550 System, NextSeq 550Dx in Research Mode, iScan System |
| Method | Methylation Array |
| Nucleic acid type | DNA |
| Number of markers |
> 935K |
| Number of samples | 8 samples per array |
| Sample throughput |
> 1000 samples per week (per Tecan for 8 sample BeadChip) |
| Sample type details |
Also compatible with buccal cells and fresh/frozen tissue |
| Specialized sample types | Blood, FFPE Tissue, Cell-Free DNA, Saliva |
| Species category | Human |
| Technology | Microarray |
| Variant class | Differentially methylated cytosines |
Requires purchase of a kit that matches the number of samples you run at a time. Please note that each kit is processed as a single batch and is not designed to be divided across multiple experiments. If you are processing several low-throughput batches, order multiple small kits. The kits contain all required reagents for performing methylation screening, except for the bisulfite conversion kits, which are optional products that can be purchased separately.
Infinium MethylationEPIC v2.0 BeadChip is a robust microarray that enables discovery of methylation-based biomarkers for research applications in cancer, genetic diseases, aging, and molecular epidemiology.
Infinium MethylationEPIC v2.0 Kit
Complex and genetic disease research
Comprehensive array and next-generation sequencing solutions to accelerate research of various genetic complex diseases.
| Infinium MethylationEPIC v2.0 Kit | Infinium Custom Methylation Kit | Infinium Mouse Methylation BeadChip |
|---|
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New and enhanced content enables future epigenetic discoveries.
CpG islands and exons that were covered insufficiently on the Infinium MethylationEPIC v1.0 BeadChip are covered thoroughly with additional probes.
FFPE tissue samples show robust performance with a modified version of the Infinium Methylation HD Assay. For precious samples and optimal sample integrity, the Infinium FFPE QC and DNA Restoration Kits are recommended.
The Infinium MethylationEPIC v2.0 BeadChip builds upon the existing CpG backbones of the Infinium MethylationEPIC v1.0 and HumanMethylation450 BeadChips.
Inf MethylationEPIC V2.0 Kit (8 Spl)
20087706
Includes one, 8-sample BeadChip and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 8 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.
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Inf MethylationEPIC V2.0 Kit (16 Spl)
20087707
Includes two, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 16 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.
List Price:
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Inf MethylationEPIC V2.0 Kit (32 Spl)
20087708
Includes four, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 32 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.
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Inf MethylationEPIC V2.0 Kit (96 Spl)
20087709
Includes 12, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 96 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.
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Inf MethylationEPIC-8+ v2.0 Kit (16 Spl)
20097750
Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 2 beadchips with reagents for processing 16 samples.
Inf MethylationEPIC-8+ v2.0 Kit (32 Spl)
20097751
Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 4 beadchips with reagents for processing 32 samples.
Inf MethylationEPIC-8+ v2.0 Kit (96 Spl)
20097752
Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 12 beadchips with reagents for processing 96 samples.
Infinium® Assay: An Introduction – Customer Site
20015273
Three-day, hands-on instruction at customer site to familiarize users with the essential steps in the Infinium protocol. Course provides hands-on training in sample and BeadChip preparation, sample scanning using the iScan System, and primary data evaluation and analysis using array analysis software.
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The Infinium Methylation EPIC v2.0 BeadChip Kit leverages powerful Infinium chemistry to detect cytosine methylation at CpG islands based on highly multiplexed genotyping of bisulfite-converted genomic DNA. Learn more about quantitative array-based methylation measurement.
This kit uses the Illumina Methylation Assay, a microarray technology that provides quantitative methylation measurement at the single-CpG-site level, powering research that examines the biological role of DNA methylation in normal and diseased cells. Learn more about the Illumina Methylation Assay.
The Infinium MethylationEPIC v2.0 BeadChip Kit contains 186K new probes that target known enhancers, super-enhancers, CTCF-binding sites, and open regions of chromatin associated with primary tumors identified by ATAC-Seq and ChIP-seq experiments. Learn about additional improvements in the Infinium MethylationEPIC v2.0 data sheet.
The GenomeStudio Methylation Module extracts beta values and provides clustering and normalization for Infinium MethylationEPIC array data. In addition, there are many freeware applications in Bioconductor, such as chAMP and SeSAMe, that provide enhanced normalization and visualization options. Illumina does not provide technical support for these freeware solutions directly. See more methylation array data analysis tips.
The Zymo EZ DNA Methylation Lightning Kit is the recommended bisulfite conversion kit for the Infinium workflow. Third party bisulfite conversion kits must be purchased separately. Learn more about validated bisulfite conversion kits for Infinium Methylation BeadChips.
The output file is an average of ~2.75 Gb with JPEG files, which is the default setting.
The scan takes ~18–37 minutes per BeadChip.
Yes, the Infinium MethylationEPIC v1.0 and HumanMethylation450 BeadChip kits have been discontinued. The Infinium MethylationEPIC v2.0 Kit, built upon the same reliable Infinium chemistry foundation, offers enhanced, expert-selected content and is the recommended replacement for both kits.
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Fully customizable methylation microarray for large, targeted epigenetic applications.
Features > 285k markers across the methylome for high-resolution epigenetic analyses of diverse murine strains.
Evaluate sample quality and repair degraded FFPE DNA samples for use in Infinium array-based assays.
A fast, integrated workflow that provides sequencing-ready DNA libraries for a wide range of targeted sequencing applications.
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