探索微生物群落在癌症治疗研究中的作用

基于NGS的研究正不断地探索宿主与微生物组的相互关系,希望能影响癌症进展和治疗有效性

癌症微生物组学研究

宿主体内的微生物会影响癌症进展和治疗有效性。饮食和药物会破坏微生物组的多样性,微生物组中的关键物种会对宿主的免疫力产生局部或全局的影响。1,2

未来的治疗方法有可能会结合现有的癌症疗法与其他能促进有益微生物生长或消除有害微生物的方法。随着基于NGS的研究继续探索宿主与微生物组的相互关系,Illumina正在努力开发可加强该领域并为其带来前景的基因组技术。

癌症免疫疗法与微生物组的作用

现代癌症免疫疗法的有效性受肠道微生物组组成的影响。

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Microbes that influence cytokine release and inflammation can exert either a positive or negative effect on tumor growth, or the ability of the immune system to suppress tumor growth.

NGS allows profiling of microbial communities in different contexts, which is critical for identifying species or conditions that may be targeted for developing new therapeutic approaches to cancer.

NGS方法彻底改变了微生物组的研究。3无需培养或克隆生物体,NGS就可同时分析微生物群落中的上千个物种。随着能管理大量新信息的生物信息学工具的发展,这种从单一生物体分析的转变能够实现物种多样性的准确评估和微生物群落动态波动的测量。

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微生物组在癌症中扮演了关键角色

本综述介绍了微生物如何影响癌症和免疫疗法,以及NGS如何推动这一不断扩展的领域的相关研究。

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The ability of NGS to analyze complex microbial communities may help to uncover new mechanisms of host–microbe interactions that promote cancer or promote drug efficacy.

The throughput and cost savings of NGS have fueled metagenomics studies capable of surveying the genomes of entire microbial communities.

NGS methods that can provide critical genetic insight into the microbiome:
Shotgun Metagenomic Sequencing

A DNA sequencing method that enables comprehensive sampling of all genes in all organisms in complex microbiome samples.

16S rRNA Sequencing

A 16S ribosomal RNA gene sequencing method used to identify and compare bacteria present within a given sample.

Microbial Metatranscriptomics

Analysis of all RNAs encoded by a group of microorganisms within a complex sample.

How Microbiome Multiomics Can Bolster Human Health

Sequencing technologies are enabling a deeper analysis of the gut's microbiome. Researchers can now explore what our microbial inhabitants are doing and how they contribute to, or protect from, diseases such as cancer and autoimmune disorders.

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Intestines with Bacteria

NGS具有极高的通量和灵敏度,可鉴定单个样本中的数千种微生物。

点击下方的查看工作流程各步骤所使用的产品。

TruSeq Stranded Total RNA Library Prep Kit

简便、经济、可扩展的总RNA分析解决方案。

Nextera XT Library Prep Kit

制备细菌、病毒和其他微生物的可直接用于测序的文库,用于分析转录组和宏转录组信息。

TruSeq DNA PCR-Free Library Preparation Kit

为全基因组测序应用提供简单全面的文库制备方案。研究人员可对多种生物进行测序,从细菌等小型基因组到人类全基因组。

桌面式测序系统
MiSeq系统

快速、准确、简便,适用于微生物学的深层次应用。

NextSeq系列

灵活的桌面式测序仪,可用于转录组和全基因组测序。

高通量测序系统

具有高通量微生物转录组学的强大功能,可根据您的项目或工作流程需要灵活改变规模。

HiSeq 4000系统

高通量、低成本,可以进行大规模基因组测序。

16S Metagenomics

利用Illumina审核的GreenGenes分类数据库对16S rRNA靶向扩增子read进行分类学分类。

Kraken宏基因组学

使用精准k-mer比对和新颖的分类算法,将分类学标签高度灵敏地快速分配给短DNA序列。

MetaPhlAn

宏基因组系统发育分析(MetaPhlAn)工具可利用鸟枪法宏基因组测序数据分析微生物群落组成。

QIIME预处理QIIME可视化

微生物生态学定量研究(QIIME)流程旨在帮助用户利用原始测序数据生成达到出版品质的图片和统计数据。

Dr. Samuel Minot from the Fred Hutchinson Cancer Research Center discusses a novel gene-level bioinformatics approach for identifying microbial species and strains associated with human diseases such as cancer, from metagenomic sequencing datasets.

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Microbiome
NGS揭示微生物的神秘世界

Microba的研究人员研究了微生物的基因组,增进了我们对人类健康、疾病和微生物进化的理解。

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NGS揭示微生物的神秘世界
Analyze Gene Expression and Chromatin Accessibility

Unify single-cell gene expression and ATAC-Seq to help reveal cellular mechanisms driving gene regulation.

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Measure Protein Expression Along with RNA-Seq

Learn how to incorporate protein detection into bulk RNA-Seq and develop a workflow for BEN-Seq, providing a holistic approach to cell analysis.

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MiSeq系统、16S rRNA测序和美国肠道计划
MiSeq系统、16S rRNA测序和美国肠道计划
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Metagenomics for Virus Discovery on Skin
HPV and Skin Cancer

Illumina sequencers offer deep coverage to identify novel HPV types correlated with non-melanoma skin cancers.

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参考文献
  1. Zama D, Biagi E, Masetti R, et al.Gut microbiota and hematopoietic stem cell transplantation: where do we stand? Bone Marrow Transplant.2017;52 (1):7-14.
  2. Schwabe R, Jobin C. The microbiome and cancer.Nat Rev Cancer.2013;13 (11):800-812.
  3. Franzosa EA, Hsu T, Sirota-Madi A, et al.Sequencing and beyond: integrating molecular 'omics' for microbial community profiling.Nat Rev Microbiol.2015;13(6):360-372.